Multiplexed High-Throughput Serological Assay for Human Enteroviruses
نویسندگان
چکیده
منابع مشابه
A high-throughput, multiplexed assay for superfamily-wide profiling of enzyme activity.
The selectivity of an enzyme inhibitor is a key determinant of its usefulness as a tool compound or its safety as a drug. Yet selectivity is never assessed comprehensively in the early stages of the drug discovery process, and only rarely in the later stages, because technical limitations prohibit doing otherwise. Here, we report EnPlex, an efficient, high-throughput method for simultaneously a...
متن کاملA high-throughput multiplexed screening assay for optimizing serum-free differentiation protocols of human embryonic stem cells.
Serum-free differentiation protocols of human embryonic stem cells (hESCs) offer the ability to maximize reproducibility and to develop clinically applicable therapies. We developed a high-throughput, 96-well plate, four-color flow cytometry-based assay to optimize differentiation media cocktails and to screen a variety of conditions. We were able to differentiate hESCs to all three primary ger...
متن کاملMultiplexed Experiment Design in High-Throughput Screening
An early step in the drug discovery involves screening through numerous chemical compounds to find molecules that have a specified biological or biochemical effect. This screening process is costly, thus any modifications to make the screen more efficient would reduce the total cost of drug discovery. Current high-throughput screening methods test each compound from a library of compounds indiv...
متن کاملA new homogeneous assay for high throughput serological diagnosis of brucellosis in ruminants.
The control and eradication of brucellosis is highly desirable but heavily resource intensive as high throughput serological testing is required. The aim of this study was to meet the needs of high throughput screening laboratories involved in this process through the development of a new assay. An existing cELISA used for the serodiagnosis of brucellosis in ruminants was converted to an AlphaL...
متن کاملHigh-throughput, multiplexed pushbroom hyperspectral microscopy.
We describe a high-throughput hyperspectral microscope. The system replaces the slit of conventional pushbroom spectral imagers with a static coded aperture mask. We present the theoretical underpinnings of the aperture coded spectral engine and describe two proof-of-concept experimental implementations. Compared to a conventional pushbroom system, the aperture coded systems have 32 times great...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microorganisms
سال: 2020
ISSN: 2076-2607
DOI: 10.3390/microorganisms8060963